Biological Indicators

Description

Biological indicators (BIs) are essential components in sterilization processes. These indicators contain living microorganisms, typically bacterial spores, that are highly resistant to the sterilization method being used. Commonly used spores include Geobacillus stearothermophilus for steam sterilization and Bacillus atrophaeus for ethylene oxide sterilization.

Biological indicators are strategically placed within a sterilization load to assess the effectiveness of the process. After exposure, these indicators are then incubated to determine whether the resistant microorganisms have been killed. If the sterilization was successful, no microbial growth should occur, providing assurance that the sterilization conditions were effective in eliminating potentially harmful microorganisms. This rigorous testing is especially critical in healthcare settings to ensure the safety of medical instruments and equipment.